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Knockdown WDHD1 suppressed cell proliferation, migration, invasion and <t>apoptosis,</t> and decreased tumor growth. (A, B) WDHD1 expression were determined by western blot (A) and RT-PCR (B) after transfection with siRNAs against WDHD1 in Hep-3B and Huh-7 cells. (C) Representative images of colony formation assay (left) and the number of colonies (right). (D) The cell proliferation assay was performed at the indicated time points. (E, F) Representative micrographs and quantitative analysis of cell migration by the transwell (E) and wound healing (F) assays. (G) Representative micrographs of cell invasion assays (left) and quantification results (right). Data are expressed as the mean ± SD of the values from three independent experiments. (H) The apoptosis was assayed using <t>Annexin</t> V FITC/PI staining. (I-K) Representative images, and weights of subcutaneous xenografts of Hep1-6 cells with WDHD1 knockdown or control. Data represent means ± SD for 5 mice per group. * P < 0.05, ** P < 0.01.
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Knockdown WDHD1 suppressed cell proliferation, migration, invasion and <t>apoptosis,</t> and decreased tumor growth. (A, B) WDHD1 expression were determined by western blot (A) and RT-PCR (B) after transfection with siRNAs against WDHD1 in Hep-3B and Huh-7 cells. (C) Representative images of colony formation assay (left) and the number of colonies (right). (D) The cell proliferation assay was performed at the indicated time points. (E, F) Representative micrographs and quantitative analysis of cell migration by the transwell (E) and wound healing (F) assays. (G) Representative micrographs of cell invasion assays (left) and quantification results (right). Data are expressed as the mean ± SD of the values from three independent experiments. (H) The apoptosis was assayed using <t>Annexin</t> V FITC/PI staining. (I-K) Representative images, and weights of subcutaneous xenografts of Hep1-6 cells with WDHD1 knockdown or control. Data represent means ± SD for 5 mice per group. * P < 0.05, ** P < 0.01.
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Knockdown WDHD1 suppressed cell proliferation, migration, invasion and <t>apoptosis,</t> and decreased tumor growth. (A, B) WDHD1 expression were determined by western blot (A) and RT-PCR (B) after transfection with siRNAs against WDHD1 in Hep-3B and Huh-7 cells. (C) Representative images of colony formation assay (left) and the number of colonies (right). (D) The cell proliferation assay was performed at the indicated time points. (E, F) Representative micrographs and quantitative analysis of cell migration by the transwell (E) and wound healing (F) assays. (G) Representative micrographs of cell invasion assays (left) and quantification results (right). Data are expressed as the mean ± SD of the values from three independent experiments. (H) The apoptosis was assayed using <t>Annexin</t> V FITC/PI staining. (I-K) Representative images, and weights of subcutaneous xenografts of Hep1-6 cells with WDHD1 knockdown or control. Data represent means ± SD for 5 mice per group. * P < 0.05, ** P < 0.01.
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Knockdown WDHD1 suppressed cell proliferation, migration, invasion and <t>apoptosis,</t> and decreased tumor growth. (A, B) WDHD1 expression were determined by western blot (A) and RT-PCR (B) after transfection with siRNAs against WDHD1 in Hep-3B and Huh-7 cells. (C) Representative images of colony formation assay (left) and the number of colonies (right). (D) The cell proliferation assay was performed at the indicated time points. (E, F) Representative micrographs and quantitative analysis of cell migration by the transwell (E) and wound healing (F) assays. (G) Representative micrographs of cell invasion assays (left) and quantification results (right). Data are expressed as the mean ± SD of the values from three independent experiments. (H) The apoptosis was assayed using <t>Annexin</t> V FITC/PI staining. (I-K) Representative images, and weights of subcutaneous xenografts of Hep1-6 cells with WDHD1 knockdown or control. Data represent means ± SD for 5 mice per group. * P < 0.05, ** P < 0.01.
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Image Search Results


Knockdown WDHD1 suppressed cell proliferation, migration, invasion and apoptosis, and decreased tumor growth. (A, B) WDHD1 expression were determined by western blot (A) and RT-PCR (B) after transfection with siRNAs against WDHD1 in Hep-3B and Huh-7 cells. (C) Representative images of colony formation assay (left) and the number of colonies (right). (D) The cell proliferation assay was performed at the indicated time points. (E, F) Representative micrographs and quantitative analysis of cell migration by the transwell (E) and wound healing (F) assays. (G) Representative micrographs of cell invasion assays (left) and quantification results (right). Data are expressed as the mean ± SD of the values from three independent experiments. (H) The apoptosis was assayed using Annexin V FITC/PI staining. (I-K) Representative images, and weights of subcutaneous xenografts of Hep1-6 cells with WDHD1 knockdown or control. Data represent means ± SD for 5 mice per group. * P < 0.05, ** P < 0.01.

Journal: Translational Oncology

Article Title: WDHD1 promotes hepatocellular carcinoma progression by affecting the cell cycle and immune evasion

doi: 10.1016/j.tranon.2026.102735

Figure Lengend Snippet: Knockdown WDHD1 suppressed cell proliferation, migration, invasion and apoptosis, and decreased tumor growth. (A, B) WDHD1 expression were determined by western blot (A) and RT-PCR (B) after transfection with siRNAs against WDHD1 in Hep-3B and Huh-7 cells. (C) Representative images of colony formation assay (left) and the number of colonies (right). (D) The cell proliferation assay was performed at the indicated time points. (E, F) Representative micrographs and quantitative analysis of cell migration by the transwell (E) and wound healing (F) assays. (G) Representative micrographs of cell invasion assays (left) and quantification results (right). Data are expressed as the mean ± SD of the values from three independent experiments. (H) The apoptosis was assayed using Annexin V FITC/PI staining. (I-K) Representative images, and weights of subcutaneous xenografts of Hep1-6 cells with WDHD1 knockdown or control. Data represent means ± SD for 5 mice per group. * P < 0.05, ** P < 0.01.

Article Snippet: Cell apoptosis was detected using the Annexin V-FITC/PI Apoptosis Detection Kit (Beyotime) according to manufacturer's instructions.

Techniques: Knockdown, Migration, Expressing, Western Blot, Reverse Transcription Polymerase Chain Reaction, Transfection, Colony Assay, Proliferation Assay, Staining, Control